CONSTRUCTION OF IMPROVED ESCHERICHIA-PSEUDOMONAS SHUTTLE VECTORS DERIVED FROM PUC18/19 AND SEQUENCE OF THE REGION REQUIRED FOR THEIR REPLICATION IN PSEUDOMONAS-AERUGINOSA

被引：441

作者：

WEST, SEH ^{[1
]}

SCHWEIZER, HP ^{[1
]}

DALL, C ^{[1
]}

SAMPLE, AK ^{[1
]}

RUNYENJANECKY, LJ ^{[1
]}

机构：

[1] UNIV CALGARY, HLTH SCI CTR, DEPT MICROBIOL & INFECT DIS, CALGARY T2N 4N1, AB, CANADA

来源：

GENE | 1994年 / 148卷 / 01期

关键词：

RECOMBINANT DNA; BROAD-HOST-RANGE EXPRESSION VECTORS; PLASMID REPLICATION; REPLICATION PROTEIN;

D O I：

10.1016/0378-1119(94)90237-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance of pMB1(ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 beta-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

引用

页码：81 / 86

页数：6

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