RATES OF CHOLESTEROL, UBIQUINONE, DOLICHOL AND DOLICHYL-P BIOSYNTHESIS IN RAT-BRAIN SLICES

被引:91
作者
ANDERSSON, M
ELMBERGER, PG
EDLUND, C
KRISTENSSON, K
DALLNER, G
机构
[1] Department of Cellular and Neuropathology, Huddinge Hospital, Karolinska Institutet
关键词
Biosynthetic rate; Brain slice; Cholesterol; Dolichol; Ubiquinone;
D O I
10.1016/0014-5793(90)81107-Y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Slices from the brain and liver of rats were prepared and upon incubation exhibited a continuous and high capacity for incorporation of radioactive precursors into proteins and lipids. Using [3H]mevalonate as precursor, the rates of biosynthesis of cholesterol, ubiquinone, dolichol and dolichyl-P in brain slices were determined and found to be 5.5,0.25,0.0093 and 0.0091 nmol/h/g, respectively. Dolichol and dolichyl-P accumulate to a limited extent, but almost all of these lipids in the brain originate from de novo synthesis. The calculated half-lives for cholesterol, ubiquinone, dolichol and dolichyl-P were 4076, 90, 1006 and 171 h, respectively. The results indicate that lipids formed via the mevalonate pathway in the brain have an active and independently regulated biosynthesis. © 1990.
引用
收藏
页码:15 / 18
页数:4
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