Homocysteine inhibits store-mediated calcium entry in human endothelial cells: Evidence for involvement of membrane potential and actin cytoskeleton

被引：1

作者：

Hong-Sheng Zhang

Jun-Hua Xiao

En-Hua Cao

Jin-Fen Qin

机构：

[1] Institute of Biophysics,Center for System Biology

[2] Huazhong University of Science & Technology,Department of Pharmacology, Tongji Medical College

[3] Institute of Biophysics,Center for System Biology

来源：

Molecular and Cellular Biochemistry | 2005年 / 269卷

关键词：

actin cytoskeleton; BK; channels; capacitative Ca; entry; homocysteine; membrane potential;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The role of homocysteine for store-operated calcium influx was investigated in human umbilical cord endothelial cell line. Homocysteine significantly decreased thapsigargin-evoked Ca2+ entry, membrane hyperpolarization and actin polymerization. GSH and DTT prevented homocysteine-induced inhibition of thapsigargin-evoked Ca2+ entry, membrane hyperpolarization and actin polymerization; while GSSG had the opposite effect. Homocysteine blocked large conductance Ca2+-activated K+ (BKCa) channels in a concentration-dependent manner and related to the redox status of the endothelial cells. BKCa channels opener NS1619 reversed thapsigargin-evoked Ca2+ entry, membrane hyperpolarization and actin polymerization; BKCa channels inhibitor iberiotoxin had the opposite effect. The findings suggest that homocysteine is involved in store-regulated Ca2+ entry through membrane potential-dependent and actin cytoskeleton-dependent mechanisms, redox status of homocysteine and BKCa channels may play a regulatory role in it. (Mol Cell Biochem 269: 37–47, 2005)

引用

页码：37 / 47

页数：10

共 142 条

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