Comparison of phenyl-type columns in the development of a fast liquid chromatographic system for eighteen opiates commonly found in forensic toxicology

被引：15

作者：

Dams, R ^{[1
]}

Lambert, WE ^{[1
]}

Clauwaert, KM ^{[1
]}

De Leenheer, AP ^{[1
]}

机构：

[1] State Univ Ghent, Toxicol Lab, B-9000 Ghent, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 896卷 / 1-2期

关键词：

forensic analysis; stationary phases; LC; opiates;

D O I：

10.1016/S0021-9673(00)00390-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a precise and reliable method for the detection of 18 of the most commonly found opiates on the Belgian legal and illicit market, by ion-exchange, reversed-phase high-performance liquid chromatography, using a conventional phenyl-type analytical column (150X4.6 mm I.D., particle size 5 mum) and diode-array detection. We also describe a performance (efficiency and sensitivity) comparison of this column to a recently developed "high-speed" column (53X7.0 mm I.D., particle size 3 mum) packed with the same stationary phase, and used under slightly adjusted how and gradient conditions. The final method, using the "high-speed" column, showed a significant reduction (55%) in analysis time without loss of resolution and sensitivity. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：311 / 319

页数：9

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