Feeling the forces: atomic force microscopy in cell biology

被引：163

作者：

Alonso, JL ^{[1
]}

Goldmann, WH ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Med, Renal Unit,Massachusetts Gen Hosp, Charlestown, MA 02129 USA

来源：

LIFE SCIENCES | 2003年 / 72卷 / 23期

关键词：

atomic force microscopy; intramolecular forces; intermolecular forces; molecular forces; cellular forces;

D O I：

10.1016/S0024-3205(03)00165-6

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Atomic force microscopy allows three-dimensional imaging and measurements of unstained and uncoated biological samples in air or fluid. Using this technology it offers resolution on the nanometer scale and detection of temporal changes in the mechanical properties, i.e. surface stiffness or elasticity in live cells and membranes. Various biological processes including ligand-receptor interactions, reorganization, and restructuring of the cytoskeleton associated with cell motility that are governed by intermolecular forces and their mode of detection will be discussed. (C) 2003 Elsevier Science Inc. All rights reserved.

引用

页码：2553 / 2560

页数：8

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