Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species - Structure-activity relationship of cloned LPA receptors

被引：236

作者：

Bandoh, K

Aoki, J

Taira, A

Tsujimoto, M

Arai, H

Inoue, K

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] RIKEN, Inst Phys & Chem Res, Lab Cellular Biochem, Wako, Saitama 3510198, Japan

来源：

FEBS LETTERS | 2000年 / 478卷 / 1-2期

关键词：

lysophosphatidic acid; endothelial cell differentiation gene family; EDG2; EDG4; EDG7;

D O I：

10.1016/S0014-5793(00)01827-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the structure-activity relationship of cloned lysophosphatidic acid (LPA) receptors (endothelial cell differentiation gene (EDG) 2, EDG4, and EDG7) by measuring [Ca2+](i) in Sf9 insect cells expressing each receptor using LPA with various acyl chains bound at either the sn-1 or the sn-2 position of the glycerol backbone, For EDG7 the highest reactivity was observed with LPA with Delta 9-unsaturated fatty acid (oleic (18:1), linoleic (18:2), and linolenic (18:3)) at sn-2 followed by 2-palmitoleoyl (16:1) and 2-arachidonoyl (20:4) LPA, In contrast, EDG2 and EDG4 showed broad ligand specificities, although EDG2 and EDG4 discriminated between 14:0 (myristoyl) and 16:0 (palmitoyl), and 12:0 (lauroyl) and 14:0 LPAs, respectively. EDG7 recognizes the cis double bond at the Delta 9 position of octadecanoyl residues, since 2-elaidoyl (18:1, tr ans) and 2 -petroselinoyl (18:1, cis-Delta 12) LPA were poor Ligands for EDG7, In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：159 / 165

页数：7

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