Genomic structure and chromosomal localization of the human GDNFR-α gene

被引：10

作者：

Eng, C

Myers, SM

Kogon, MD

Sanicola, M

Hession, C

Cate, RL

Mulligan, LM

机构：

[1] Dana Farber Canc Inst, Charles A Dana Human Canc Genet Unit, Richard & Sara Smith Labs, Boston, MA 02115 USA

[2] Univ Cambridge, CRC, Human Canc Genet Res Grp, Cambridge CB2 2QQ, England

[3] Queens Univ, Dept Pathol, Kingston, ON K7L 3N6, Canada

[4] Queens Univ, Dept Paediat, Kingston, ON K7L 3N6, Canada

[5] Biogen Inc, Dept Mol Biol, Cambridge, MA 02142 USA

来源：

ONCOGENE | 1998年 / 16卷 / 05期

基金：

英国医学研究理事会;

关键词：

RET; GFR alpha-1; GDNF; Exon-intron; 10q;

D O I：

10.1038/sj.onc.1201573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GDNFR-alpha is a glycosyl-phosphotidylinositol-linked receptor for glial cell line-derived neurotrophic factor (GDNF), GDNF binds to GDNFR-alpha and this complex, in turn, is believed to interact with the RET receptor tyrosine kinase to effect downstream signalling. GDNFR-alpha belongs to a novel gene family without strong homology to known genes. Thus, little information has been available to help predict genomic structure or location of this gene. In this study, the genomic organization of human GDNFR-alpha was delineated through a combination of PAC clone characterization, long distance PCR and sequence analyses. Exon-intron boundaries were defined by comparing the size and sequence of the genomic PCR products to those predicted by the cDNA sequence. The human GDNFR-alpha gene comprises 9 exons. GDNFR-alpha PAC clones were used for FISH analysis to map this gene to 10q26.

引用

页码：597 / 601

页数：5

共 31 条

[1]

Ausubel FM, 1995, CURRENT PROTOCOLS MO

[2] TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret [J].

Baloh, RH ;

Tansey, MG ;

Golden, JP ;

Creedon, DJ ;

Heuckeroth, RO ;

Keck, CL ;

Zimonjic, DB ;

Popescu, NC ;

Johnson, EM ;

Milbrandt, J .

NEURON, 1997, 18 (05) :793-802

[3]

BOLINO A, 1995, ONCOGENE, V10, P2415

[4] Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase [J].

BujBello, A ;

Adu, J ;

Pinon, LGP ;

Horton, A ;

Thompson, J ;

Rosenthal, A ;

Chinchetru, M ;

Buchman, VL ;

Davies, AM .

NATURE, 1997, 387 (6634) :721-724

[5] SINGLE MISSENSE MUTATION IN THE TYROSINE KINASE CATALYTIC DOMAIN OF THE RET PROTOONCOGENE IS ASSOCIATED WITH MULTIPLE ENDOCRINE NEOPLASIA TYPE 2B [J].

CARLSON, KM ;

DOU, SS ;

CHI, D ;

SCAVARDA, N ;

TOSHIMA, K ;

JACKSON, CE ;

WELLS, SA ;

GOODFELLOW, PJ ;

DONISKELLER, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1579-1583

[6] MUTATIONS IN THE RET PROTOONCOGENE ARE ASSOCIATED WITH MEN 2A AND FMTC [J].

DONISKELLER, H ;

DOU, SS ;

CHI, D ;

CARLSON, KM ;

TOSHIMA, K ;

LAIRMORE, TC ;

HOWE, JR ;

MOLEY, JF ;

GOODFELLOW, P ;

WELLS, SA .

HUMAN MOLECULAR GENETICS, 1993, 2 (07) :851-856

[7] GDNF signalling through the Ret receptor tyrosine kinase [J].

Durbec, P ;

MarcosGutierrez, CV ;

Kilkenny, C ;

Grigoriou, M ;

Wartiovaara, K ;

Suvanto, P ;

Smith, D ;

Ponder, B ;

Costantini, F ;

Saarma, M ;

Sariola, H ;

Pachnis, V .

NATURE, 1996, 381 (6585) :789-793

[8] POINT MUTATION WITHIN THE TYROSINE KINASE DOMAIN OF THE RET PROTOONCOGENE IN MULTIPLE ENDOCRINE NEOPLASIA TYPE 2B AND RELATED SPORADIC TUMORS [J].

ENG, C ;

SMITH, DP ;

MULLIGAN, LM ;

NAGAI, MA ;

HEALEY, CS ;

PONDER, MA ;

GARDNER, E ;

SCHEUMANN, GFW ;

JACKSON, CE ;

TUNNACLIFFE, A ;

PONDER, BAJ .

HUMAN MOLECULAR GENETICS, 1994, 3 (02) :237-241

[9]

ENG C, 1995, ONCOGENE, V10, P509

[10]

Eng C, 1997, HUM MUTAT, V9, P97, DOI 10.1002/(SICI)1098-1004(1997)9:2<97::AID-HUMU1>3.3.CO

← 1 2 3 4 →