Sequencing and high level expression in Escherichia coli of the tropomyosin allergen (Der p 10) from Dermatophagoides pteronyssinus

被引：74

作者：

Asturias, JA ^{[1
]}

Arilla, MC ^{[1
]}

Gómez-Bayón, N ^{[1
]}

Martínez, A ^{[1
]}

Martínez, J ^{[1
]}

Palacios, R ^{[1
]}

机构：

[1] IFIDESA, ARISTEGUI, Dept Res & Dev, Bilbao 48008, Spain

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1397卷 / 01期

关键词：

allergy; IgE antibody; pan allergen; recombinant allergen; (dust mite);

D O I：

10.1016/S0167-4781(98)00006-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients. (C) 1998 Elsevier Science B.V.

引用

页码：27 / 30

页数：4

共 22 条

[1] IMMUNOCHEMICAL CHARACTERIZATION OF RECOMBINANT AND NATIVE TROPOMYOSINS AS A NEW ALLERGEN FROM THE HOUSE-DUST MITE, DERMATOPHAGOIDES-FARINAE [J].

AKI, T ;

KODAMA, T ;

FUJIKAWA, A ;

MIURA, K ;

SHIGETA, S ;

WADA, T ;

JYO, T ;

MUROOKA, Y ;

OKA, S ;

ONO, K .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 96 (01) :74-83

[2] Cloning and expression of the panallergen profilin and the major allergen (Ole e 1) from olive tree pollen [J].

Asturias, JA ;

Arilla, MC ;

GomezBayon, N ;

Martinez, J ;

Martinez, A ;

Palacios, R .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 100 (03) :365-372

[3] DETECTION OF IGE ANTIBODIES TO A WIDE-RANGE OF INSECT SPECIES IN SUBJECTS WITH SUSPECTED INHALANT ALLERGIES TO INSECTS [J].

BALDO, BA ;

PANZANI, RC .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1988, 85 (03) :278-287

[4]

BUSCH RK, 1996, IMMUNOL ALLERGY CLIN, V16, P535

[5]

DEMAATBLEEKER F, 1995, ALLERGY, V50, P438, DOI 10.1111/j.1398-9995.1995.tb01174.x

[6] IgE reactivity against a cross-reactive allergen in crustacea and mollusca: Evidence fev tropomyosin as the common allergen [J].

Leung, PSC ;

Chow, WK ;

Duffey, S ;

Kwan, HS ;

Gershwin, ME ;

Chu, KH .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1996, 98 (05) :954-961

[7]

Martinez A, 1997, Allergol Immunopathol (Madr), V25, P118

[8] COMPARISON OF THE LEVELS OF THE MAJOR ALLERGENS DER-P-I AND DER-P-II IN STANDARDIZED EXTRACTS OF THE HOUSE-DUST MITE, DERMATOPHAGOIDES-PTERONYSSINUS [J].

MEYER, CH ;

BOND, JF ;

CHEN, MS ;

KASAIAN, MT .

CLINICAL AND EXPERIMENTAL ALLERGY, 1994, 24 (11) :1041-1048

[9] CLONING AND CHARACTERIZATION OF A MAJOR ALLERGEN OF THE HOUSE-DUST MITE, DERMATOPHAGOIDES-PTERONYSSINUS, HOMOLOGOUS WITH GLUTATHIONE-S-TRANSFERASE [J].

ONEILL, GM ;

DONOVAN, GR ;

BALDO, BA .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1219 (02) :521-528

[10] DUST MITE ALLERGENS AND ASTHMA - REPORT OF A 2ND INTERNATIONAL WORKSHOP [J].

PLATTSMILLS, TAE ;

THOMAS, WR ;

AALBERSE, RC ;

VERVLOET, D ;

CHAPMAN, MD ;

BISCHOFF, E ;

BRUIJNZEELKOOMEN, CAFM ;

CHARPIN, D ;

COLLOFF, MJ ;

EGGLESTON, PA ;

EHNERT, B ;

GOLDSTEIN, RA ;

VANHAGEHAMSTEN, M ;

HART, BJ ;

HOLGATE, ST ;

HONG, CS ;

JOHANSSON, SGO ;

KORSGAARD, J ;

LAU, S ;

LEMAO, J ;

LOWENSTEIN, H ;

MERRETT, TG ;

MITCHELL, EB ;

MIYAMOTO, T ;

MUDDE, GC ;

NASPITZ, CK ;

OHEHIR, RE ;

OKUDAIRA, H ;

PAULI, G ;

POLLART, SM ;

REED, CE ;

REES, J ;

RING, J ;

ROMAGNANI, S ;

SCHOU, C ;

SEARS, MR ;

SPIEKSMA, FTM ;

STEWART, GA ;

THOMAS, WR ;

TOVEY, ER ;

TURNER, KJ ;

VERVLOET, D ;

VIELUF, D ;

DEWECK, AL ;

WEN, T ;

WAHN, U ;

WOOLCOCK, AJ ;

YASUEDA, H ;

YOUNG, R .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (05) :1046-1060

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