Central delivery of human tissue kallikrein gene reduces blood pressure in hypertensive rats

被引：12

作者：

Wang, C

Chao, C

Madeddu, P

Chao, L

Chao, J ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Biochem & Mol Biol, Charleston, SC 29425 USA

[2] Univ Sassari, Med Clin, I-07100 Sassari, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 244卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8232

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human tissue kallikrein gene, in the form of naked DNA (CMV-cHK) or an adenoviral vector (Ad.CMV-cHK), was directly delivered by intracerebroventricular injection into spontaneously hypertensive rats. Control rats received the same amount of vector DNA (pcDNA(3)) or adenoviral vector (Ad.CMV-LacZ) carrying the lacZ gene. A single injection of the human tissue kallikrein gene caused a rapid and prolonged blood pressure-lowering effect that; began 1 day post injection and the effect lasted for more than 7 days. The expression of human tissue kallikrein and its mRNA was identified in the cortex, cerebellum, brain stem, hippocampus and hypothalamus. Cellular localization of beta-galactosidase was detected by X-gal staining in the thalamus, hypothalamus and third ventricle in rats injected with Ad.CMV-LacZ. This suggests that the tissue kallikrein-kinin system may function in the central control of blood pressure homeostasis. (C) 1998 Academic Press.

引用

页码：449 / 454

页数：6

共 26 条

[1]

Berecek K H, 1984, J Hypertens Suppl, V2, pS63

[2]

BHOOLA KD, 1992, PHARMACOL REV, V44, P1

[3] TISSUE KALLIKREIN IN RAT-BRAIN AND PITUITARY - REGIONAL DISTRIBUTION AND ESTROGEN INDUCTION IN THE ANTERIOR-PITUITARY [J].