Establishment of the mimetic aging effect in mice caused by D-galactose

被引:430
作者
Ho, SC [1 ]
Liu, JH [1 ]
Wu, RYY [1 ]
机构
[1] Dev Ctr Biotechnol, Tradit Chinese Med Div, Taipei, Taiwan
关键词
D-dalactose; free radical; malondialdehyde; mimetic aging model; superoxide dismutase; total antioxidant status;
D O I
10.1023/A:1022417102206
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
We successfully established an oxidant damage of mimetic aging model using mice induced by D-galactose, and the mimetic aging model is relative to free radical and the accumulation of waste substances in metabolism. The animals were divided into 3 groups: (1) phosphate-buffered saline (PBS); (2) 1 % D-galactose; (3) 5 % D-galactose by subcutaneous injection every day. After 45 days, mice treated with D-galactose showed a significant increase in the malondialdehyde (MDA), total antioxidant status (TAS) and a decrease in superoxide dismutase (SOD) in the blood compared with the PBS group. In the brain, the D-galactose treated mice exhibited a higher level MDA and a lower level SOD activity. In the liver, only the 5% D-galactose group indicated a significant increase in MDA. By reference to the oxidative biomarkers in blood, brain and liver, we have confirmed the reliability of the mimetic aging model.
引用
收藏
页码:15 / 18
页数:4
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