Structural insights into DNA polymerase β fidelity:: hold tight if you want it right

被引：83

作者：

Beard, WA ^{[1
]}

Wilson, SH ^{[1
]}

机构：

[1] NIEHS, Struct Biol Lab, Res Triangle Pk, NC 27709 USA

来源：

CHEMISTRY & BIOLOGY | 1998年 / 5卷 / 01期

关键词：

D O I：

10.1016/S1074-5521(98)90081-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA polymerases must select and incorporate the correct deoxynucleoside 5'-triphosphate from a pool of structurally similar molecules. The structural and kinetic characterization of DNA polymerase beta indicates that this polymerase must stabilize the templating base to achieve efficient polymerization with high fidelity.

引用

收藏

页码：R7 / R13

页数：7

相关论文

共 27 条

[1] DNA polymerase beta: Structure-fidelity relationship from pre-steady-state kinetic analyses of all possible correct and incorrect base pairs for wild type and R283A mutant [J].

Ahn, J ;

Werneburg, BG ;

Tsai, MD .

BIOCHEMISTRY, 1997, 36 (05) :1100-1107

[2] Enzyme-DNA interactions required for efficient nucleotide incorporation and discrimination in human DNA polymerase beta [J].

Beard, WA ;

Osheroff, WP ;

Prasad, R ;

Sawaya, MR ;

Jaju, M ;

Wood, TG ;

Kraut, J ;

Kunkel, TA ;

Wilson, SH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (21) :12141-12144

[3]

Beard WA, 1995, METHOD ENZYMOL, V262, P98

[4] A minor groove binding track in reverse transcriptase [J].

Bebenek, K ;

Beard, WA ;

Darden, TA ;

Li, LP ;

Prasad, R ;

Luxon, BA ;

Gorenstein, DG ;

Wilson, SH ;

Kunkel, TA .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) :194-197

[5] Increased activity and fidelity of DNA polymerase beta on single-nucleotide gapped DNA [J].

Chagovetz, AM ;

Sweasy, JB ;

Preston, BD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (44) :27501-27504

[6]

ECHOLS H, 1991, ANNU REV BIOCHEM, V60, P477, DOI 10.1146/annurev.biochem.60.1.477

[7]

Efrati E, 1997, J BIOL CHEM, V272, P2559

[8] Hydrogen bonding revisited: Geometric selection as a principal determinant of DNA replication fidelity [J].

Goodman, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10493-10495

[9] Replication of template-primers containing propanodeoxyguanosine by DNA polymerase beta - Induction of base pair substitution and frameshift mutations by template slippage and deoxynucleoside triphosphate stabilization [J].

Hashim, MF ;

SchnetzBoutaud, N ;

Marnett, LJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (32) :20205-20212

[10]

HUNTER WN, 1987, J BIOL CHEM, V262, P9962