Interleukin-5 mRNA expressed by eosinophils and gamma/delta T cells in parasite-immune sheep

Interleukin (IL)-5 is produced by a variety of cell types and contributes to both lymphocyte development and eosinophil terminal differentiation in vitro. The coincidence of worm expulsion and eosinophilia in sheep infected with the gastrointestinal nematode Trichostrongylus colubriformis suggests that eosinophils may be involved as effector cells in host immunity against parasite infection. The role of IL-5 in this process was investigated by observing the distribution of IL-5 mRNA(+) cells in the small intestine, mesenteric lymph nodes (MLN) and Peyer`s parches (PP) by an in situ hybridization technique using a murine IL-5 riboprobe. IL-5 mRNA(+) cells were distributed throughout the lamina propria (LP) of the small intestine from the tips of the villi to the muscularis mucosae and in the parafollicular areas of MLN and PP in both naive and immune sheep. The phenotypes of IL-5 mRNA(+) cells was explored by simultaneous eosin and immunohistochemical staining using a monoclonal antibody recognizing the T19 marker. which identifies a major subset of gamma/delta TCR(-) cells in sheep. In immune sheep. there was about a five-fold increase in the number of eosinophils and IL-5 mRNA(-) cells in the LP, but there was no significant change in numbers of T19(-) cells. Most of the IL-5 mRNA(-) cells in the LP were eosinophils, but many of the T19 cells also expressed IL-5 mRNA. In contrast, there were fewer eosinophils than T19(-) cells in MLN of immune sheep and, compared to controls, a three-fold increase in T19(+) cells and a five-fold increase in T19(-)/IL-5 mRNA(-) double-positive cells was observed in immune sheep. In PP, there were very few eosinophils but substantial numbers of T19(+) cells; however, no significant differences in numbers of eosinophils, T19(+) or IL-5 mRNA(-) cells were observed between control and immune sheep. These results indicate that in sheep, both eosinophils and gamma/delta T cells are capable of IL-5 expression and suggest that IL-5 is an important regulatory factor in autocrine and paracrine activation of effector cells involved in parasite immune expulsion.