Green fluorescent protein as a scaffold for intracellular presentation of peptides

被引：123

作者：

Abedi, MR ^{[1
]}

Caponigro, G ^{[1
]}

Kamb, A ^{[1
]}

机构：

[1] Ventana Genet Inc, Salt Lake City, UT 84108 USA

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 02期

关键词：

D O I：

10.1093/nar/26.2.623

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide aptamers provide probes for biological processes and adjuncts for development of novel pharmaceutical molecules, Such aptamers are analogous to compounds derived from combinatorial chemical libraries which have specific binding or inhibitory activities. Much as it is generally difficult to determine the composition of combinatorial chemical libraries in a quantitative manner, determining the quality and characteristics of peptide libraries displayed in vivo is problematical. To help address these issues we have adapted green fluorescent protein (GFP) as a scaffold for display of conformationally constrained peptides, The GFP-peptide libraries permit analysis of library diversity and expression levels in cells and allow enrichment of the libraries for sequences with predetermined characteristics, such as high expression of correctly folded protein, by selection for high fluorescence.

引用

页码：623 / 630

页数：8

共 19 条

[1] Modulation of Ras and a-factor function by carboxyl-terminal proteolysis [J].