Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells

The present study identifies the phosphorylation sites of the 85-kDa cytosolic phospholipase A(2) (cPLA(2)) in human platelets and HeLa cells, Tryptic digests of P-32-phosphorylated and -immunoprecipitated cPLA(2) were analyzed by microbore high performance liquid chromatography and two-dimensional phosphopeptide mapping against synthetic phosphopeptide standards, Thrombin stimulated significant phosphorylation of platelet cPLA(2) at two sites, Ser-505 and Ser-727. Exclusive phosphorylation on these two sites was also seen in collagen-stimulated platelets and HeLa cells stimulated with interferon-alpha or arsenite; no tyrosine phosphorylation was detected. The inhibitor of the 38-kDa stress-activated protein kinase (p38(mapk)), SB 203580, reduced phosphorylation of both Ser-505 and Ser-727 by 50 and 60%, respectively, in thrombin-stimulated platelets. An additional p38(mapk) inhibitor SB 202190 also partially (60%) inhibited the phosphorylation of cPLA(2) in arsenite-stimulated HeLa cells, These studies extend the previous work on the identification of multiple phosphorylation sites on cPLA(2) expressed in a baculovirus/insect cell system to cPLA(2) in mammalian cells stimulated with physiological agonists, They also underscore the necessity of high resolution phosphopeptide mapping combined with microbore high performance liquid chromatography for quantification of phosphorylation levels, which has lead to the conclusion that Ser-505 and Ser-727 are common phosphorylation sites on cPLA(2) in different mammalian cells stimulated with multiple agonists.