The role of pp60(c-src) in the regulation of calcium entry via store-operated calcium channels

In many cell types, G protein-coupled receptors stimulate a transient Ca2+ release from internal stores followed by a sustained, capacitative Ca2+ entry, which is mediated by store-operated channels (SOCs), Although it is clear that SOCs are activated by depletion of internal Ca2+ stores, the mechanism for this process is not well understood, Previously, we have reported that inhibitors of tyrosine kinase activity block the bradykinin-and thapsigarin-stimulated Ca2+ entry in fibroblasts, suggesting that a tyrosine kinase activity may be involved in relaying the message from the empty internal Ca2+ stores to the plasma membrane Ca2+ channel (Lee, K.-M., Toscas, K,, and Villereal, RI, L, (1993) J, Biol, Chem, 268, 9945-9948), We also have demonstrated that bradykinin activates the nonreceptor tyrosine kinase c-src (Lee, K.-M., and Villereal, hi. L, (1996) Am. J, Physiol. 270, C1430-C1437). We investigated whether c-src plays a role in the regulation of SOCs by monitoring capacitative Ca2+ entry in 3T3-like embryonic fibroblast lines derived from either wild type or src(-)/src(-) (Src(-)) transgenic mice, We report that Ca2+ entry, following store depletion by either bradykinin or thapsigargin, is dramatically lower in Src-fibroblasts than in wild type fibroblasts. The level of capacitative Ca2+ entry in Src(-) cells is restored to nearly normal levels by transfecting Src-cells with chicken c-src, These data suggest that c-src may play a major role in the regulation of SOCs.