Cis1/Atg31 is required for autophagosome formation in Saccharomyces cereviside

被引：82

作者：

Kabeya, Yukiko

Kawarnata, Tomoko

Suzuki, Kuninori

Ohsumi, Yoshinori

机构：

[1] Natl Inst Basic Biol, Div Mol Cell Biol, Okazaki, Aichi 4448585, Japan

[2] Grad Univ Adv Studies, Sch Life Sci, Okazaki, Aichi 4448585, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 356卷 / 02期

关键词：

autophagy; starvation; ATG; autophagosorne; PAS; rapamycin;

D O I：

10.1016/j.bbrc.2007.02.150

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autophagy is the bulk degradation of cytosolic materials in lysosomes/vacuoles of eukaryotic cells. In the yeast SaccharomYces cerevisiae, 17 Atg proteins are known to be involved in autophagosome formation. Genorne wide analyses have shown that Atgl7 interacts with numerous proteins. Further studies on these interacting proteins may provide further insights into membrane dynamics during autophagy. Here, we identify Cisl/Atg3l as a protein that exhibits similar phenotypes to Atg17. ATG31 null cells were defective in aUtophagy and lost viability under starvation conditions. Localization of Atg3l to p re-a utophago soma I structures (PAS) was dependent on Atgl7. Coirnmunoprecipitation experiments indicated that Atg3l interacts with Atgl7. Together, Atg31 is a novel protein that, in concert with Atgl7, is required for proper autophagosome formation. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：405 / 410

页数：6

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