Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets

被引：9775

作者：

Lewis, BP

Burge, CB

Bartel, DP

机构：

[1] MIT, Dept Biol, Cambridge, MA 02139 USA

[2] Whitehead Inst Biomed Res, Cambridge Ctr 9, Cambridge, MA 02142 USA

来源：

CELL | 2005年 / 120卷 / 01期

关键词：

D O I：

10.1016/j.cell.2004.12.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of our gene set. Targeting was also detected in open reading frames. In sum, well over one third of human genes appear to be conserved miRNA targets.

引用

页码：15 / 20

页数：6

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