A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13

被引：90

作者：

AnsariLari, MA

Muzny, DM

Lu, J

Lu, F

Lilley, CE

Spanos, S

Malley, T

Gibbs, RA

机构：

[1] Dept. of Molec. and Human Genetics, Baylor College of Medicine, Houston

来源：

GENOME RESEARCH | 1996年 / 6卷 / 04期

关键词：

D O I：

10.1101/gr.6.4.314

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genomic sequence of the human CD4 gene and its neighboring region, located at chromosome 12p13, was generated using the large-scale shotgun sequencing strategy. A total of 117 kb of genomic sequence and similar to 11 kb of cDNA sequence were obtained. Six gents, including CD4, triosephosphate isomerase, B3 subunit Of G proteins (GNB3), and ubiquitin isopeptidase T (ISOT), with known functions, and two new genes with unknown functions were identified. Using a battery of strategies, the exon/intron boundaries, splice variants, and tissue expression patterns of the genes were determined. Various computer software was utilized for analyses of the DNA and amino acid sequences. The results of the analyses and sequence-based strategies for gene identification are discussed.

引用

页码：314 / 326

页数：13

共 40 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] ADAPTER-BASED URACIL DNA GLYCOSYLASE CLONING SIMPLIFIES SHOTGUN LIBRARY CONSTRUCTION FOR LARGE-SCALE SEQUENCING [J].