The new frontier of genome engineering with CRISPR-Cas9

被引：4561

作者：

Doudna, Jennifer A. ^{[1
,2
,3
]}

Charpentier, Emmanuelle ^{[4
,5
,6
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[4] Helmholtz Ctr Infect Res, Dept Regulat Infect Biol, D-38124 Braunschweig, Germany

[5] Umea Univ, Lab Mol Infect Med Sweden, Umea Ctr Microbial Res, Dept Mol Biol, S-90187 Umea, Sweden

[6] Hannover Med Sch, D-30625 Hannover, Germany

来源：

SCIENCE | 2014年 / 346卷 / 6213期

基金：

瑞典研究理事会;

关键词：

RNA-GUIDED CAS9; CRISPR/CAS9-MEDIATED TARGETED MUTAGENESIS; SITE-SPECIFIC CLEAVAGE; DOUBLE-STRAND BREAKS; ONE-STEP GENERATION; HOMOLOGOUS RECOMBINATION; DNA RECOGNITION; HUMAN-CELLS; INTERFERENCE COMPLEX; CRYSTAL-STRUCTURE;

D O I：

10.1126/science.1258096

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.

引用

页码：1077 / +

页数：10

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