The genome of the diatom Thalassiosira pseudonana:: Ecology, evolution, and metabolism

被引：1500

作者：

Armbrust, EV ^{[1
]}

Berges, JA

Bowler, C

Green, BR

Martinez, D

Putnam, NH

Zhou, SG

Allen, AE

Apt, KE

Bechner, M

Brzezinski, MA

Chaal, BK

Chiovitti, A

Davis, AK

Demarest, MS

Detter, JC

Glavina, T

Goodstein, D

Hadi, MZ

Hellsten, U

Hildebrand, M

Jenkins, BD

Jurka, J

Kapitonov, VV

Kröger, N

Lau, WWY

Lane, TW

Larimer, FW

Lippmeier, JC

Lucas, S

Medina, M

Montsant, A

Obornik, M

Parker, MS

Palenik, B

Pazour, GJ

Richardson, PM

Rynearson, TA

Saito, MA

Schwartz, DC

Thamatrakoln, K

Valentin, K

Vardi, A

Wilkerson, FP

Rokhsar, DS

机构：

[1] Univ Washington, Sch Oceanog, Seattle, WA 98195 USA

[2] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA

[3] Stn Zool, Plant Mol Biol Lab, I-80121 Naples, Italy

[4] Ecole Normale Super, Dept Biol, F-75230 Paris, France

[5] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada

[6] Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA

[7] Univ Wisconsin, Dept Genet, Madison, WI 53706 USA

[8] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

[9] Princeton Univ, Dept Geosci, Princeton, NJ 08540 USA

[10] Martek Biosci Corp, Columbia, MD 21045 USA

[11] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA

[12] Univ Calif Santa Barbara, Inst Marine Sci, Santa Barbara, CA 93106 USA

[13] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia

[14] Univ Calif San Diego, Scripps Inst Oceanog, La Jolla, CA 92093 USA

[15] Sandia Natl Labs, Lockheed Martin Corp, Livermore, CA 94551 USA

[16] Univ Calif Santa Cruz, Ocean Sci Dept, Santa Cruz, CA 95064 USA

[17] Genet Informat Res Inst, Mountain View, CA 94043 USA

[18] Univ Regensburg, Lehrstuhl Biochem 1, D-93053 Regensburg, Germany

[19] Sandia Natl Labs, Biosyst Res Dept, Livermore, CA 94551 USA

[20] Oak Ridge Natl Lab, Genome Anal Grp, Oak Ridge, TN 37831 USA

[21] Univ Hull, Dept Biol Sci, Kingston Upon Hull HU6 7RX, N Humberside, England

[22] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[23] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA

[24] Alfred Wegener Inst Polar & Marine Res, D-27570 Bremerhaven, Germany

[25] San Francisco State Univ, Romberg Tiburon Ctr, Tiburon, CA 94920 USA

[26] Univ Calif Berkeley, Ctr Integrat Genom, Berkeley, CA 94720 USA

来源：

SCIENCE | 2004年 / 306卷 / 5693期

关键词：

D O I：

10.1126/science.1101156

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Diatoms are unicellular algae with plastids acquired by secondary endosymbiosis. They are responsible for similar to20% of global carbon fixation. We report the 34 million-base pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair ptastid and 44 thousand-base pair mitochondrial genomes. Sequence and optical restriction mapping revealed 24 diploid nuclear chromosomes. We identified novel genes for silicic acid transport and formation of silica-based cell walls, high-affinity iron uptake, biosynthetic enzymes for several types of polyunsaturated fatty acids, use of a range of nitrogenous compounds, and a complete urea cycle, all attributes that allow diatoms to prosper in aquatic environments.

引用

页码：79 / 86

页数：8

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