HKCNMB3 and hKCNMB4, cloning and characterization of two members of the large-conductance calcium-activated potassium channel β subunit family

被引：256

作者：

Behrens, R ^{[1
]}

Nolting, A ^{[1
]}

Reimann, F ^{[1
]}

Schwarz, M ^{[1
]}

Waldschütz, R ^{[1
]}

Pongs, O ^{[1
]}

机构：

[1] Univ Hamburg, ZMNH, Inst Neurale Signalverarbeitung, D-20246 Hamburg, Germany

来源：

FEBS LETTERS | 2000年 / 474卷 / 01期

关键词：

potassium channel; calcium-activated; accessory subunit; expression pattern;

D O I：

10.1016/S0014-5793(00)01584-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned two beta subunits of large-conductance calcium-activated potassium (BK) channels, hKCNMB3 (BK beta 1) and hKCNMB4 (BK beta 4). Profiling mRNA expression showed that hKCNMB3 expression is enriched in testis and hKCNMB4 expression is very prominent in brain. We coexpressed BK channel alpha (BK alpha) and BK beta 4 subunits in vitro in CHO cells. We compared BK alpha/beta 4 mediated currents with those of smooth muscle BK alpha/beta 1 channels. BK beta 4 slowed activation kinetics more significantly, led to a steeper apparent calcium sensitivity, and shifted the voltage range of BK current activation to more negative potentials than BK beta 1. BK alpha/beta 4 channels were not blocked by 100 nM charybdotoxin or iberiotoxin, and were activated by 17 beta-estradiol. (C) 2000 Federation of European Biochemical Societies.

引用

页码：99 / 106

页数：8

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