Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

被引：208

作者：

Aapola, U

Shibuya, K

Scott, HS

Ollila, J

Vihinen, M

Heino, M

Shintani, A

Kawasaki, K

Minoshima, S

Krohn, K

Antonarakis, SE

Shimizu, N

Kudoh, J

Peterson, P

机构：

[1] Univ Tampere, Inst Med Technol, Dept Pathol, FIN-33101 Tampere, Finland

[2] Tampere Univ Hosp, Tampere 33520, Finland

[3] Keio Univ, Sch Med, Dept Mol Biol, Shinjuku Ku, Tokyo 1608582, Japan

[4] Univ Geneva, Sch Med, Div Med Genet, CH-1211 Geneva, Switzerland

[5] Univ Hosp Geneva, Div Med Genet, Geneva, Switzerland

[6] Univ Helsinki, Dept Biosci, Div Biochem, FIN-00014 Helsinki, Finland

来源：

GENOMICS | 2000年 / 65卷 / 03期

基金：

日本学术振兴会; 芬兰科学院; 日本科学技术振兴机构;

关键词：

D O I：

10.1006/geno.2000.6168

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.

引用

页码：293 / 298

页数：6

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