3-hydroxybenzoate:coenzyme a ligase and 4-coumarate: Coenzyme A ligase from cultured cells of Centaurium erythraea

被引：17

作者：

Barillas, W ^{[1
]}

Beerhues, L ^{[1
]}

机构：

[1] UNIV BONN,INST PHARMAZEUT BIOL,D-53115 BONN,GERMANY

来源：

PLANTA | 1997年 / 202卷 / 01期

关键词：

Centaurium (cell cultures); coenzyme A ligases (3-hydroxybenzoate:CoA ligase; 4-coumarate:CoA ligase); xanthone biosynthesis (enzymology);

D O I：

10.1007/s004250050109

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

3-Hydroxybenzoate:coenzyme A ligase, an enzyme involved in xanthone biosynthesis; Tvas detected in cell-free extracts from cultured cells of Centaurium erythraea Rafn, The enzyme was separated from 4 coumarate:coenzyme A ligase by fractionated ammonium sulphate precipitation and hydrophobic interaction chromatography. The CoA ligases exhibited different substrate specificities. 3-Hydroxybenzoate:coenzyme A ligase activated 3-hydroxybenzoic acid most efficiently and lacked affinity for cinnamic acids, In contrast, 4-coumarate:CoA ligase mainly catalyzed the activation of 4-coumaric acid but did not act on benzoic acids. The two enzymes were similar with respect to their relative molecular weight, their pH and temperature optimal their specific activity and the changes in their activity during cell culture growth.

引用

页码：112 / 116

页数：5

共 26 条

[1] Benzophenone synthase from cultured cells of Centaurium erythraea [J].