Structure and expression of the promoter for the R4/ALK5 human type I transforming growth factor-beta receptor: Regulation by TGF-beta

被引：52

作者：

Bloom, BB ^{[1
]}

Humphries, DE ^{[1
]}

Kuang, PP ^{[1
]}

Fine, A ^{[1
]}

Goldstein, RH ^{[1
]}

机构：

[1] VET ADM MED CTR,BOSTON,MA 02118

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1996年 / 1312卷 / 03期

关键词：

transforming growth factor-beta; receptor; promoter; human lung fibroblast;

D O I：

10.1016/0167-4889(96)00043-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type I transforming growth factor-beta (TGF-beta) receptors are serine/threonine kinases that are essential for the action of TGF-beta. In this paper, we describe the molecular cloning and expression of the R4/ALK5 human type I TGF-beta receptor promoter. DNA sequence analysis indicates that the promoter lacks a TATA and CAAT box but is highly GC-rich and contains putative Sp1 binding sites. The transcriptional start site is approx. 232 base pairs upstream of the AUG start codon. In human lung fibroblasts, TGF-beta induced a 3-fold increase in steady-state level for type I receptor mRNA. Exposure of cells transfected with a 618 bp promoter fragment to TGF-beta 1 up-regulated transcriptional activity indicating that a TGF-beta response element is contained within this region.

引用

页码：243 / 248

页数：6

共 39 条

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