DEC1 negatively regulates AMPK activity via LKB1

被引：30

作者：

Sato, Fuyuki ^{[1
,2
,3
]}

Muragaki, Yasuteru ^{[3
]}

Zhang, Yanping ^{[1
,2
,4
]}

机构：

[1] Univ N Carolina, Sch Med, Dept Radiat Oncol, Chapel Hill, NC 27514 USA

[2] Univ N Carolina, Sch Med, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27514 USA

[3] Wakayama Med Univ, Sch Med, Dept Pathol, Wakayama 6418509, Japan

[4] Xuzhou Med Coll, Inst Canc, Jiangsu Ctr Collaborat & Innovat Canc Biotherapy, Xuzhou 221002, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 467卷 / 04期

关键词：

DEC1; AMPK; LKB1; Clock gene; TRANSCRIPTION FACTOR DEC1; METABOLIC SYNDROME; CIRCADIAN-RHYTHMS; E-BOX; EXPRESSION; LOOP; CLOCK; PATHWAY; CANCER; GENE;

D O I：

10.1016/j.bbrc.2015.10.077

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Basic helix-loop-helix (bHLH) transcription factor DEC1 (bHLHE40/Stra13/Sharp2) is one of the clock genes that show a circadian rhythm in various tissues. AMP-activated protein kinase (AMPK) activity plays important roles in the metabolic process and in cell death induced by glucose depletion. Recent reports have shown that AMPK activity exhibited a circadian rhythm. However, little is known regarding the regulatory mechanisms involved in the circadian rhythm of AMPK activity. The aim of this study is to investigate whether there is a direct correlation between DEC1 expression and AMPK activity. DEC1 protein and AMPK activity showed a circadian rhythm in the mouse liver with different peak levels. Knocking down DEC1 expression increased AMPK activity, whereas overexpression of DEC1 decreased it. Overexpressing the DEC1 basic mutants had little effect on the AMPK activity. DEC1 bound to the E-box of the LKB1 promoter, decreased LKB1 activity and total protein levels. There was an inverse relationship between DEC1 expression and AMPK activity. Our results suggest that DEC1 negatively regulates AMPK activity via LKB1. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：711 / 716

页数：6

共 40 条

[1] Mice Lacking the Circadian Modulators SHARP1 and SHARP2 Display Altered Sleep and Mixed State Endophenotypes of Psychiatric Disorders [J].

Baier, Paul C. ;

Brzozka, Magdalena M. ;

Shahmoradi, Ali ;

Reinecke, Lisa ;

Kroos, Christina ;

Wichert, Sven P. ;

Oster, Henrik ;

Wehr, Michael C. ;

Taneja, Reshma ;

Hirrlinger, Johannes ;

Rossner, Moritz J. .

PLOS ONE, 2014, 9 (10)

[2] Interdependence of AMPK and SIRT1 for Metabolic Adaptation to Fasting and Exercise in Skeletal Muscle [J].

Canto, Carles ;

Jiang, Lake Q. ;

Deshmukh, Atul S. ;

Mataki, Chikage ;

Coste, Agnes ;

Lagouge, Marie ;

Zierath, Juleen R. ;

Auwerx, Johan .

CELL METABOLISM, 2010, 11 (03) :213-219

[3] Dysfunctional AMPK activity, signalling through mTOR and survival in response to energetic stress in LKB1-deficient lung cancer [J].

Carretero, J. ;

Medina, P. P. ;

Blanco, R. ;

Smit, L. ;

Tang, M. ;

Roncador, G. ;

Maestre, L. ;

Conde, E. ;

Lopez-Rios, F. ;

Clevers, H. C. ;

Sanchez-Cespedes, M. .

ONCOGENE, 2007, 26 (11) :1616-1625

[4] AMPK: a nutrient and energy sensor that maintains energy homeostasis [J].

Hardie, D. Grahame ;

Ross, Fiona A. ;

Hawley, Simon A. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2012, 13 (04) :251-262

[5] The AMP-activated protein kinase - Fuel gauge of the mammalian cell? [J].

Hardie, DG ;

Carling, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 246 (02) :259-273

[6]

Hawley Simon A, 2003, J Biol, V2, P28, DOI 10.1186/1475-4924-2-28

[7] The Transcriptional Repressor DEC2 Regulates Sleep Length in Mammals [J].

He, Ying ;

Jones, Christopher R. ;

Fujiki, Nobuhiro ;

Xu, Ying ;

Guo, Bin ;

Holder, Jimmy L., Jr. ;

Rossner, Moritz J. ;

Nishino, Seiji ;

Fu, Ying-Hui .

SCIENCE, 2009, 325 (5942) :866-870

[8] Dec1 and Dec2 are regulators of the mammalian molecular clock [J].

Honma, S ;

Kawamoto, T ;

Takagi, Y ;

Fujimoto, K ;

Sato, F ;

Noshiro, M ;

Kato, Y ;

Honma, K .

NATURE, 2002, 419 (6909) :841-+

[9] A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation [J].

Kawamoto, T ;

Noshiro, M ;

Sato, F ;

Maemura, K ;

Takeda, N ;

Nagai, R ;

Iwata, T ;

Fujimoto, K ;

Furukawa, M ;

Miyazaki, K ;

Honma, S ;

Honma, K ;

Kato, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 313 (01) :117-124

[10] Effects of fasting and re-feeding on the expression of Dec1, Per1, and other clock-related genes [J].

Kawamoto, Takeshi ;

Noshiro, Mitsuhide ;

Furukawa, Masae ;

Honda, Kiyomasa K. ;

Nakashima, Ayumu ;

Ueshima, Taichi ;

Usui, Emiko ;

Katsura, Yuki ;

Fujimoto, Katsumi ;

Honma, Sato ;

Honma, Ken-ichi ;

Hamada, Taizo ;

Kato, Yukio .

JOURNAL OF BIOCHEMISTRY, 2006, 140 (03) :401-408

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