Glutathione content and adaptation to endogenously induced energy depletion in Mv1Lu cells

被引：4

作者：

Boada, J ^{[1
]}

Cuesta, E ^{[1
]}

Perales, JC ^{[1
]}

Rom, T ^{[1
]}

Bermudez, J ^{[1
]}

机构：

[1] Univ Barcelona, Dept Ciencies Fisiol 2, E-08907 Barcelona, Spain

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 2004年 / 36卷 / 12期

关键词：

metabolic stress; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; fructose 2,6-bisphosphate; glutathione Na+-K+ pump; potassium channels; protein synthesis; free radicals;

D O I：

10.1016/j.freeradbiomed.2004.04.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transfection of genes that code for enzymes of energy metabolism provides alternative models to study the adaptive response to energy restriction induced by endogenous changes instead of by unfavorable environmental conditions. Overexpression of the glycolytic enzyme fructose-2,6-bisphosphatase reduced the content of fructose 2,6-bisphosphate, inducing energy limitation in the mink lung epithelial cell line Mv1Lu. This metabolic stress reduced the ATP available in transfected cells by 20%, which downregulated active ion transport and protein turnover. Ion homeostasis and cell function require concomitant reductions in cell membrane ion permeability and protein damage. Our results indicate that glutathione content linked these features of the adaptive response to the endogenously induced metabolic downregulation. (C) 2004 Elsevier Inc. All rights reserved.

引用

收藏

页码：1555 / 1565

页数：11

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