Distribution of orexin/hypocretin in the rat median eminence and pituitary

被引：119

作者：

Date, Y

Mondal, MS

Matsukura, S

Ueta, Y

Yamashita, H

Kaiya, H

Kangawa, K

Nakazato, M ^{[1
]}

机构：

[1] Miyazaki Med Coll, Dept Internal Med 3, Miyazaki 8891692, Japan

[2] Natl Cardiovasc Ctr, Res Inst, Osaka 5658565, Japan

[3] Univ Occupat & Environm Hlth, Sch Med, Dept Physiol, Kitakyushu, Fukuoka 8078555, Japan

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 76卷 / 01期

关键词：

orexin; hypocretin; orexin receptor; pituitary; immunohistochemistry; in situ hybridization;

D O I：

10.1016/S0169-328X(99)00317-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We determined the: distribution of orexin-A and orexin-B (also known as hypocretin-1 and hypocretin-2) and their receptors in the rat median eminence and pituitary using sensitive radioimmunoassays coupled with high-performance liquid chromatography, immunohistochemistry, and in situ hybridization. Orexin-A and -B were present in the median eminence, adenohypophysis, and neurohypophysis. Orexin fibers were abundant in the median eminence, and a few fibers projected to the neurohypophysis. Both the orexin(1)- and orexin(2)-receptor mRNAs were expressed robustly in the pituitary intermediate lube, whereas in the anterior lobe, the orexin(1) receptor was more markedly expressed than the orexin(2) receptor. These: two receptor mRNAs were also found in the posterior lube. These findings may implicate orexin's involvement in additional as yet undefined physiological functions in the hypothalamo-hypophyseal tract. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 6

页数：6

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