The use of heterologously expressed drug metabolizing enzymes - state of the art and prospects for the future

被引：112

作者：

Crespi, CL ^{[1
]}

Miller, VP ^{[1
]}

机构：

[1] GENTEST Corp, Woburn, MA 01801 USA

来源：

PHARMACOLOGY & THERAPEUTICS | 1999年 / 84卷 / 02期

关键词：

cytochrome P450; drug metabolism; drug interactions; hepatic; liver;

D O I：

10.1016/S0163-7258(99)00028-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The first report of the functional, heterologous expression of a mammalian cytochrome P450 (CYP) enzyme occurred more than a decade ago. In the intervening years, these expression systems have been optimized with regard to the specific requirements for production of catalytically active enzymes. In this review, we discuss the strengths and limitations of heterologously expressed enzymes as they affect in vitro drug metabolism studies. Emphasis is given to new applications (screens for CYP inhibition and novel enzyme mixtures) that have been enabled by high level, functional expression of CYP enzymes. (C) 1999 Elsevier Science Inc. All rights reserved.

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页码：121 / 131

页数：11

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