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Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

被引：1583
作者：
Bae, Sangsu [1 ,2 ]
Park, Jeongbin [3 ]
Kim, Jin-Soo [1 ,2 ]
机构：
[1] Seoul Natl Univ, Natl Creat Res Initiat, Ctr Genome Engn, Seoul 151742, South Korea
[2] Seoul Natl Univ, Dept Chem, Seoul 151742, South Korea
[3] Seoul Natl Univ, Dept Phys & Astron, Seoul 151742, South Korea
来源：
BIOINFORMATICS | 2014年 / 30卷 / 10期
基金：
新加坡国家研究基金会;
关键词：
ZINC-FINGER NUCLEASES; HUMAN GENOME; HUMAN-CELLS; SEQUENCES;
D O I：
10.1093/bioinformatics/btu048
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The Type II clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system is an adaptive immune response in prokaryotes, protecting host cells against invading phages or plasmids by cleaving these foreign DNA species in a targeted manner. CRISPR/Cas-derived RNA-guided engineered nucleases (RGENs) enable genome editing in cultured cells, animals and plants, but are limited by off-target mutations. Here, we present a novel algorithm termed Cas-OFFinder that searches for potential off-target sites in a given genome or user-defined sequences. Unlike other algorithms currently available for identification of RGEN off-target sites, Cas-OFFinder is not limited by the number of mismatches and allows variations in protospacer-adjacent motif sequences recognized by Cas9, the essential protein component in RGENs. Cas-OFFinder is available as a command-line program or accessible via our website.
引用
收藏
页码：1473 / 1475
页数：3
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