Comparison of different methods for plant regeneration and transformation of the legume Galega orientalis Lam. (goat's rue)

被引：16

作者：

Collén, AMC ^{[1
]}

Jarl, CI ^{[1
]}

机构：

[1] Lund Univ, S-22007 Lund, Sweden

来源：

PLANT CELL REPORTS | 1999年 / 19卷 / 01期

关键词：

Agrobacterium; particle bombardment; whole-plant electroporation;

D O I：

10.1007/s002990050703

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

For the first time, regeneration and transformation have been achieved from the legume Galega orientalis Lam. (goat's rue). Two different regeneration protocols are described, one based on direct shoot induction from meristems and the other :involving callus induction and shoot induction from callus with the plant growth regulator thidiazuron (TDZ). Different media and explants were evaluated. Three different transformation methods were compared: cocultivation with four different Agrobacterium tumefaciens strains, electroporation of embryos and apical meristems and particle bombardment of embryos. TDZ-promoted shoot induction on calli from immature embryos gave the best results. Transformation using this regeneration protocol was most successful with particle bombardment. Stable transformation has yet to be proven.

引用

页码：13 / 19

页数：7

共 27 条

[1]

Atkins C. A., 1997, Biological fixation of nitrogen for ecology and sustainable agriculture., P283

[2] ADVENTITIOUS SHOOT PRODUCTION FROM IMMATURE EMBRYOS OF WHITE CLOVER [J].