Analysis of the Escherichia coli Tol-Pal and TonB systems by periplasmic production of Tol, TonB, colicin, or phage capsid soluble domains

被引：37

作者：

Bouveret, E ^{[1
]}

Journet, L ^{[1
]}

Walburger, A ^{[1
]}

Cascales, E ^{[1
]}

Bénédetti, H ^{[1
]}

Lloubès, R ^{[1
]}

机构：

[1] CNRS, LISM, F-13402 Marseille 20, France

来源：

BIOCHIMIE | 2002年 / 84卷 / 5-6期

关键词：

Tol-Pal; TonB; colicins; G3p; translocation domain; protein interaction;

D O I：

10.1016/S0300-9084(02)01423-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this review is to describe an in vivo assay of the interactions taking place in the Tol-Pal or TonB-ExbB-ExbD envelope complexes in the periplasm of Escherichia coli and between them and colicins or g3p protein of filamentous bacteriophages. Domains of colicins or periplasmic soluble domains of Tol or TonB proteins can be artificially addressed to the periplasm of bacteria by fusing them to a signal sequence from an exported protein. These domains interact specifically in the periplasm with the Tol or TonB complexes and disturb their function, which can be directly detected by the appearance of specific tol or tonB phenotypes. This technique can be used to detect new interactions, to characterize them biochemically and to map them or to induce tol or tonB phenotypes to study the functions of these two complexes. 0 2002 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.

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页码：413 / 421

页数：9

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