Prion protein (PrP) is not involved in the pathogenesis of spongiform encephalopathy in transgenic mice expressing interleukin-6 in the brain

被引：3

作者：

Castelnau, PA

Campbell, IL

Powell, HC

机构：

[1] VET AFFAIRS RES SERV, LA JOLLA, CA USA

[2] Scripps Res Inst, DEPT NEUROPHARMACOL, LA JOLLA, CA USA

来源：

NEUROSCIENCE LETTERS | 1997年 / 234卷 / 01期

关键词：

prion protein; glia; swelling; spongiosis; degeneration; blood-brain barrier; cytokine; transgenic;

D O I：

10.1016/S0304-3940(97)00670-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Transgenic mice expressing interleukin-6 (IL6) in the brain exhibit gliosis, spongiosis and neuronal loss. Based on previous findings, we hypothesized that IL6 could upregulate the prion protein (PrP) gene in the central nervous system (CNS) of these mice. Western and Northern blot analysis showed that PrP protein and mRNA levels were comparable to control levels. Furthermore, ultrastructural characterization revealed that spongiosis was actually located in astrocytes. These results indicate that IL6 does not upregulate the cerebral PrP expression in this animal model and that profound astrocytic alterations precipitate the neuronal degeneration observed. (C) 1997 Elsevier Science Ireland Ltd.

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页码：15 / 18

页数：4

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