Serum MicroRNAs Are Promising Novel Biomarkers

被引：1110

作者：

Gilad, Shlomit ^{[1
]}

Meiri, Eti ^{[1
]}

Yogev, Yariv ^{[2
]}

Benjamin, Sima ^{[1
]}

Lebanony, Danit ^{[1
]}

Yerushalmi, Noga ^{[1
]}

Benjamin, Hila ^{[1
]}

Kushnir, Michal ^{[1
]}

Cholakh, Hila ^{[1
]}

Melamed, Nir ^{[2
]}

Bentwich, Zvi ^{[1
]}

Hod, Moshe ^{[2
]}

Goren, Yaron ^{[1
]}

Chajut, Ayelet ^{[1
]}

机构：

[1] Rosetta Genom Ltd, Rehovot, Israel

[2] Tel Aviv Univ, Sackler Fac Med, Rabin Med Ctr, Div Maternal Fetal Med, IL-49100 Petah Tiqwa, Israel

来源：

PLOS ONE | 2008年 / 3卷 / 09期

关键词：

D O I：

10.1371/journal.pone.0003148

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. Methods and Results: After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. Conclusions and Significance: Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers.

引用

页数：7

共 24 条

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