Towards single-copy gene expression systems making gene cloning physiologically relevant:: Lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system

被引：146

作者：

Boyd, D ^{[1
]}

Weiss, DS ^{[1
]}

Chen, JC ^{[1
]}

Beckwith, J ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA

来源：

JOURNAL OF BACTERIOLOGY | 2000年 / 182卷 / 03期

关键词：

D O I：

10.1128/JB.182.3.842-847.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a simple system for reversible, stable integration of plasmid-borne genes into the Escherichia coli chromosome. Most ordinary E. coli strains and a variety of pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, a lambda phage, is the only specialized vector required. The resultant strains have a single copy of the plasmid fragment inserted stably at the lambda attachment site on the chromosome, with nearly the entire lambda genome deleted.

引用

页码：842 / 847

页数：6

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