Highly sensitive method to detect mRNAs in individual cells by direct RT-PCR using Tth DNA polymerase

被引：14

作者：

Chiocchia, G ^{[1
]}

Smith, KA ^{[1
]}

机构：

[1] NEW YORK HOSP,CORNELL MED CTR,NEW YORK,NY 10021

来源：

BIOTECHNIQUES | 1997年 / 22卷 / 02期

关键词：

D O I：

10.2144/97222st04

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for detecting the expression of low-abundance mRNA molecules has been developed that combines the sensitivity of PCR, the high efficiency and specificity of reverse transcription (RT) using Tth DNA polymerase at high temperature, and the enhancement of sensitivity and specificity of nested PCR. This method is highly sensitive, reproducible and allows the detection of MRNAs in individual cells by direct RT-PCR.

引用

页码：312 / &

页数：4

共 8 条

[1] FUNCTIONAL ISOLATION AND CHARACTERIZATION OF HUMAN HEMATOPOIETIC STEM-CELLS [J].