Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex

被引：197

作者：

OwenHughes, T

Utley, RT

Cote, J

Peterson, CL

Workman, JL

机构：

[1] PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,UNIVERSITY PK,PA 16802

[2] UNIV MASSACHUSETTS,MED CTR,DEPT BIOCHEM & MOLEC BIOL,WORCESTER,MA 01605

[3] UNIV MASSACHUSETTS,MED CTR,PROGRAM MOLEC MED,WORCESTER,MA 01605

来源：

SCIENCE | 1996年 / 273卷 / 5274期

关键词：

D O I：

10.1126/science.273.5274.513

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The SWI/SNF complex participates in the restructuring of chromatin for transcription. The function of the yeast SWI/SNF complex in the remodeling oi a nucleosome array has now been analyzed in vitro. Binding of the purified SWI/SNF complex to a nucleosome array disrupted multiple nucleosomes in an adenosine triphosphate-dependent reaction. However, removal of SWI/SNF left a deoxyribonuclease I-hypersensitive site specifically at a nucleosome that was bound by derivatives of the transcription factor Gal4p. Analysis of individual nucleosomes revealed that the SWI/SNF complex catalyzed eviction of histones from the Gal4-bound nucleosomes. Thus, the transient action of the SWI/SNF complex facilitated irreversible disruption of transcription factor-bound nucleosomes.

引用

页码：513 / 516

页数：4

共 35 条

[1] THE TRANSCRIPTIONALLY-ACTIVE MMTV PROMOTER IS DEPLETED OF HISTONE H1 [J].