Antimicrobial efficacy of copper-doped titanium surfaces for dental implants

被引:35
作者
Astasov-Frauenhoffer, Monika [1 ]
Koegel, Sally [1 ]
Waltimo, Tuomas [1 ]
Zimmermann, Andrea [2 ]
Walker, Cyril [2 ]
Hauser-Gerspach, Irmgard [1 ]
Jung, Christiane [2 ]
机构
[1] Univ Basel, Univ Ctr Dent Med, Dept Oral Hlth & Med, Hebelstr 3, CH-4056 Basel, Switzerland
[2] KKS Ultraschall AG, Med Surface Ctr, Frauholzring 29, CH-6422 Steinen, Switzerland
关键词
ANTIBACTERIAL ACTIVITY; ION-IMPLANTATION; COATINGS; SILVER; STRATEGIES; RESISTANCE; METALS;
D O I
10.1007/s10856-019-6286-y
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
The aim of this in vitro study was to quantify the antibacterial effect of a copper-deposited titanium surface as a model for dental implants on the peri-implantitis-associated strain Porphyromonas gingivalis (DSM 20709). A spark-assisted anodization method in a combined deposition-anodization process was applied to deposit copper on discs made of titanium. This method allows the deposition of different concentrations of copper on the surface by varying the process time. Conventional culturing was used to investigate the adhesion of P. gingivalis onto the discs over 2, 4, and 6h as well as to study the antibacterial effect of copper released in solution. The viability of the bacterial cells is strongly inhibited on copper-deposited discs and reaches a CFU reduction of 3 log-units after 6h in comparison to the reference. The copper released in solution causes a reduction of 4 log-units after a 6h incubation time. With a 6h incubation time, the CFU count decreases with increasing copper concentrations on the disc (by 2% for the 1.3 mu g/disc; 32% for the 5.6 mu g/disc; and 34% for the 9.5 mu g/disc). However, at a higher copper concentration of 17.7 mu g/disc, after 6h, the decrease in the CFU count is less pronounced than that observed in solution, where a further decrease is observed. In conclusion, copper-functionalized titanium significantly reduces the survival of adhered bacteria and decreases the viable bacterial count in the environment surrounding the titanium. Thus, the area surrounding implants is being protected by copper released from the surface, forming a safe zone for improved implant healing. [GRAPHICS] .
引用
收藏
页数:9
相关论文
共 43 条
[1]   Eugenol-induced suppression of biofilm-forming genes in Streptococcus mutans: An approach to inhibit biofilms [J].
Adil, Mohd ;
Singh, Kunal ;
Verma, Praveen K. ;
Khan, Asad U. .
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2014, 2 (04) :286-292
[2]  
[Anonymous], 2018, J ADV TRANSPORT
[3]  
[Anonymous], EUR CELLS MAT
[4]   The anti-bacterial activity of titanium-copper sintered alloy against Porphyromonas gingivalis in vitro [J].
Bai, Bing ;
Zhang, Erlin ;
Liu, Junchao ;
Zhu, Jingtao .
DENTAL MATERIALS JOURNAL, 2016, 35 (04) :659-667
[5]  
Berry C W, 1992, Implant Dent, V1, P59, DOI 10.1097/00008505-199200110-00006
[6]   DEPENDENCE OF APATITE FORMATION ON SILICA-GEL ON ITS STRUCTURE - EFFECT OF HEAT-TREATMENT [J].
CHO, SB ;
NAKANISHI, K ;
KOKUBO, T ;
SOGA, N ;
OHTSUKI, C ;
NAKAMURA, T ;
KITSUGI, T ;
YAMAMURO, T .
JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 1995, 78 (07) :1769-1774
[7]   The increasing use of silver-based products as antimicrobial agents: a useful development or a cause for concern? [J].
Chopra, Ian .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 59 (04) :587-590
[8]   Reducing implant loss rates in immediate breast reconstructions [J].
Darragh, L. ;
Robb, A. ;
Hardie, C. M. ;
McDonald, S. ;
Valand, P. ;
O'Donogue, J. M. .
BREAST, 2017, 31 :208-213
[9]   COPPER-INHIBITION OF THE GROWTH OF ORAL STREPTOCOCCI AND ACTINOMYCES [J].
DUGUID, R .
BIOMATERIALS, 1983, 4 (03) :225-227
[10]   Characterisation of discharge events during plasma electrolytic oxidation [J].
Dunleavy, C. S. ;
Golosnoy, I. O. ;
Curran, J. A. ;
Clyne, T. W. .
SURFACE & COATINGS TECHNOLOGY, 2009, 203 (22) :3410-3419