Radionuclide imaging of mesenchymal stem cells transplanted into spinal cord

被引：14

作者：

Bai, JZ

Ding, WIIM

Yu, MJ

Du, J

Liu, ZJ

Jia, B

Li, LS

Shen, L

Tian, JH

Wang, F ^{[1
]}

机构：

[1] Peking Univ, Med Isotopes Res Ctr, Beijing 100083, Peoples R China

[2] Peking Univ, Hosp 3, Dept Orthoped, Beijing 100083, Peoples R China

[3] Chinese Peoples Liberat Army Gen Hosp, Dept Nucl Med, Beijing 100853, Peoples R China

[4] Peking Univ, Stem Cell Res Ctr, Beijing 100083, Peoples R China

来源：

NEUROREPORT | 2004年 / 15卷 / 07期

关键词：

in vivo tracking; mesenchymal stem cell; radionuclide imaging; spinal cord; transferrin receptor; transplantation;

D O I：

10.1097/00001756-200405190-00007

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In vivo tracking of stem cells implanted to spinal cord by radionuclide imaging was investigated. The high expression of transferrin receptor on human mesenchymal stem cells (hMSCs) was verified by flow cytometry, radioligand binding and immunofluorescence. Radiolabelled transferrin was chosen as a tracer for scintigraphic imaging of the hMSCs transplanted into spinal cord of rabbits. Comparative experiments with radiolabelled human serum albumin as tracer and PBS as graft as well as ex vivo autoradiography demonstrated the specific uptake of radiolabelled transferrin of hMSCs. hMSCs could be detected in vivo with radiolabelled transferrin targeting at cellular transferrin receptors at an early stage after transplantation into spinal cord.

引用

页码：1117 / 1120

页数：4

共 16 条

[1] Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells [J].