CD14 mediates endotoxin induction of nitric oxide synthase in cultured brain glial cells

被引:73
作者
Galea, E
Reis, DJ
Fox, ES
Xu, H
Feinstein, DL
机构
[1] CORNELL UNIV,COLL MED,DEPT NEUROL & NEUROSCI,DIV NEUROBIOL,NEW YORK,NY 10021
[2] ST LOUIS UNIV,CARDINAL GLENNON CHILDRENS HOSP,HLTH SCI CTR,PEDIAT RES INST,ST LOUIS,MO 63110
关键词
astrocytes; microglia; lipopolysaccharide; nitrites; cDNA; mRNA;
D O I
10.1016/0165-5728(95)00143-3
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Here we show that lipopolysaccharide (LPS) induction of glial inducible nitric oxide synthase (iNOS) requires membrane (m) and soluble (s) forms of CD14. In glial cell cultures, an anti-rat CD14 monoclonal antibody detected CD14 protein in whole cells and cell lysates, and reduced LPS-dependent iNOS expression. Glial cells and normal brain tissue expressed CD14 mRNA, as revealed by isolation of a rat CD14 clone (rCD14) from an astrocyte cDNA library, and RT-PCR analysis. Finally, serum reduced the ED(50) of LPS required for glial iNOS expression, and antibodies against sCD14 blocked the potentiating effect of serum.
引用
收藏
页码:19 / 28
页数:10
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