Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas

被引：178

作者：

Chen, YT ^{[1
]}

Stockert, E ^{[1
]}

Jungbluth, A ^{[1
]}

Tsang, SL ^{[1
]}

Coplan, KA ^{[1
]}

Scanlan, MJ ^{[1
]}

Old, LJ ^{[1
]}

机构：

[1] NEW YORK HOSP,CORNELL MED CTR,DEPT PATHOL,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 12期

关键词：

tumor immunology; cancer vaccine;

D O I：

10.1073/pnas.93.12.5915

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent progress in the structural identification of human melanoma antigens recognized by autologous cytotoxic T cells has led to the recognition of a new melanocyte differentiation antigen, Melan-A(MART-1). To determine the properties of the Melan-A gene product, Melan-A recombinant protein was produced in Escherichia coli and used to generate mouse monoclonal antibodies (mAbs). Two prototype mAhs, A103 and A355, were selected for detailed study. Immunoblotting results with A103 showed a 20-22-kDa doublet in Melan-A mRNA positive melanoma cell lines and no reactivity with Melan-A mRNA-negative cell lines. A355, in addition to the 20-22-kDa doublet, recognized several other protein species in Melan-A mRNA-positive cell lines. Immunocytochemical assays on cultured melanoma cells showed specific and uniform cytoplasmic staining in Melan-A mRNA-positive cell lines, Immunohistochemical analysis of normal human tissues with both mAbs showed staining of adult melanocytes and no reactivity with the other normal tissues tested, Analysis of 21 melanoma specimens showed homogenous staining of tumor cell cytoplasm in 16 of 17 Melan-A mRNA-positive cases and no reactivity with the three Melan-A mRNA-negative cases.

引用

页码：5915 / 5919

页数：5

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