Iterative In Situ Click Chemistry Creates Antibody-like Protein-Capture Agents

被引：97

作者：

Agnew, Heather D. ^{[1
]}

Rohde, Rosemary D. ^{[1
]}

Millward, Steven W. ^{[1
]}

Nag, Arundhati ^{[1
]}

Yeo, Woon-Seok ^{[1
]}

Hein, Jason E. ^{[2
,3
]}

Pitram, Suresh M. ^{[2
,3
]}

Tariq, Abdul Ahad ^{[1
]}

Burns, Vanessa M. ^{[1
]}

Krom, Russell J. ^{[1
]}

Fokin, Valery V. ^{[2
,3
]}

Sharpless, K. Barry ^{[2
,3
]}

Heath, James R. ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 27期

基金：

加拿大自然科学与工程研究理事会;

关键词：

click chemistry; combinatorial chemistry; inhibitors; peptides; protein-capture agents; CARBONIC-ANHYDRASE; APTAMER; INHIBITORS; ACETYLCHOLINESTERASE; HYDROLYSIS; MOLECULES; LIBRARIES; SELECTION; VITRO; ACIDS;

D O I：

10.1002/anie.200900488

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4944 / 4948

页数：5

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