Comparative analysis of the ribosomal components of the hydrogenosome-containing protist, Trichomonas vaginalis

被引:3
作者
Arisue, N
Maki, Y
Yoshida, H
Wada, A
Sánchez, LB
Müller, M
Hashimoto, T [1 ]
机构
[1] Grad Univ Adv Studies Sokendai, Dept Biosyst Sci, Hayama, Kanagawa 2400193, Japan
[2] Osaka Med Coll, Dept Phys, Takatsuki, Osaka 5690084, Japan
[3] Rockefeller Univ, New York, NY 10021 USA
[4] Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan
关键词
ribosome; sedimentation coefficient; ribosomal protein; rRNA; Trichomonas vaginalis;
D O I
10.1007/s00239-004-2604-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ribosomes of the amitochondriate but hydrogenosome-containing protist lineage, the trichomonads, have previously been reported to be prokaryotic or primitive eukaryotic, based on evidence that they have a 70S sedimentation coefficient and a small number of proteins, similar to prokaryotic ribosomes. In order to determine whether the components of the trichomonad ribosome indeed differ from those of typical eukaryotic ribosomes, the ribosome of a representative trichomonad, Trichomonas vaginalis, was characterized. The sedimentation coefficient of the T. vaginalis ribosome was smaller than that of Saccharomyces cerevisiae and larger than that of Eschrichia coli. Based on two-dimensional PAGE analysis, the number of different ribosomal proteins was estimated to be approximately 80. This number is the same as those obtained for typical eukaryotes (approximately 80) but larger than that of E. coli (approximately 55). N-Terminal amino acid sequencing of 18 protein spots and the complete sequences of 4. ribosomal proteins as deduced from their genes revealed these sequences to display typical eukaryotic features. Phylogenetic analyses of the five ribosomal proteins currently available also clearly confirmed that the T. vaginalis sequences are positioned within a eukaryotic clade. Comparison of deduced secondary structure models of the small and large subunit rRNAs of T. vaginalis with those of other eukaryotes revealed that all helices commonly found in typical eukaryotes are present and conserved in T. vaginalis, while variable regions are shortened or lost. These lines of evidence demonstrate that the T. vaginalis ribosome has no prokaryotic or primitive eukaryotic features but is clearly a typical eukaryotic type.
引用
收藏
页码:59 / 71
页数:13
相关论文
共 79 条
[1]  
[Anonymous], 1996, COMPUTER SCI MONOGRA
[2]   The phylogenetic position of the pelobiont Mastigamoeba balamuthi based on sequences of rDNA and translation elongation factors EF-1α and EF-2 [J].
Arisue, N ;
Hashimoto, T ;
Lee, JA ;
Moore, DV ;
Gordon, P ;
Sensen, CW ;
Gaasterland, T ;
Hasegawa, M ;
Müller, M .
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2002, 49 (01) :1-10
[3]   The analysis of 100 genes supports the grouping of three highly divergent amoebae:: Dictyostelium, Entamoeba, and Mastigamoeba [J].
Bapteste, E ;
Brinkmann, H ;
Lee, JA ;
Moore, DV ;
Sensen, CW ;
Gordon, P ;
Duruflé, L ;
Gaasterland, T ;
Lopez, P ;
Müller, M ;
Philippe, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1414-1419
[4]   The phagotrophic origin of eukaryotes and phylogenetic classification of protozoa [J].
Cavalier-Smith, T .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2002, 52 :297-354
[5]   KINGDOM PROTOZOA AND ITS 18 PHYLA [J].
CAVALIERSMITH, T .
MICROBIOLOGICAL REVIEWS, 1993, 57 (04) :953-994
[6]   MOLECULAR EVOLUTION - EUKARYOTES WITH NO MITOCHONDRIA [J].
CAVALIERSMITH, T .
NATURE, 1987, 326 (6111) :332-333
[7]   SUCROSE DENSITY GRADIENT SEDIMENTATION OF ESCHERICHIA-COLI RIBOSOMES [J].
CHAIRES, JB ;
KEGELES, G .
BIOPHYSICAL CHEMISTRY, 1977, 7 (03) :173-178
[8]   CHARACTERIZATION OF THE RDNA UNIT AND SEQUENCE-ANALYSIS OF THE SMALL SUBUNIT RIBOSOMAL-RNA AND 5.8S RIBOSOMAL-RNA GENES FROM TRITRICHOMONAS-FETUS [J].
CHAKRABARTI, D ;
DAME, JB ;
GUTELL, RR ;
YOWELL, CA .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 52 (01) :75-84
[9]   RIBOSOMES FROM TRICHOMONAD PROTOZOA HAVE PROKARYOTIC CHARACTERISTICS [J].
CHAMPNEY, WS ;
CHITTUM, HS ;
SAMUELS, R .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1992, 24 (07) :1125-1133
[10]   Direct amplification of unknown genes and fragments by Uneven polymerase chain reaction [J].
Chen, XF ;
Wu, R .
GENE, 1997, 185 (02) :195-199