Sequencing, expression and biochemical characterization of the Porphyromonas gingivalis pepO gene encoding a protein homologous to human endothelin-converting enzyme

被引：31

作者：

Awano, S

Ansai, T ^{[1
]}

Mochizuki, H

Yu, WX

Tanzawa, K

Turner, AJ

Takehara, T

机构：

[1] Kyushu Dent Coll, Dept Prevent Dent, Kokurakita Ku, Kitakyushu, Fukuoka 8038580, Japan

[2] Sankyo Co Ltd, Biol Res Labs, Shinagawa Ku, Tokyo 1408710, Japan

[3] Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

FEBS LETTERS | 1999年 / 460卷 / 01期

关键词：

protease; neutral endopeptidase; endothelin; endothelin-converting enzyme; Porphyromonas gingivalis;

D O I：

10.1016/S0014-5793(99)01326-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the nucleotide sequence of the clone pAL2 obtained from Porphyromonas gingivalis 381 in the previous study [Ansai ct al, (1995) Microbiology 141, 2047-2052], The DNA sequence analysis of this fragment revealed one complete ORF and one incomplete ORF, The ORF encoded a protein (PgPepO) of 690 amino acids with a calculated molecular weight of 78796, The deduced amino acid sequence exhibited a significant homology with human endothelin-converting enzyme (ECE)-1. Recombinant PgPepO was purified to homogeneity and characterized. The purified enzyme was strongly inhibited by phosphoramidon, and converted big endothelin-1 to endothelin-1. Furthermore, the purified PgPepO strongly cross-reacted with a monoclonal antibody against rat ECE-1, These results indicate that PgPepO has striking similarity to mammalian ECE in structure and function. (C) 1999 Federation of European Biochemical Societies.

引用

页码：139 / 144

页数：6

共 33 条

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