OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin κ genes

被引：61

作者：

Casellas, R

Jankovic, M

Meyer, G

Gazumyan, A

Luo, Y

Roeder, RG

Nussenzweig, MC ^{[1
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA

[2] Rockefeller Univ, Lab Mol Immunol, New York, NY 10021 USA

[3] Rockefeller Univ, Biochem & Mol Biol Lab, New York, NY 10021 USA

来源：

CELL | 2002年 / 110卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(02)00911-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

OcaB, a transcriptional coactivator also known as Bob-1 or OBF-1, was isolated on the basis of its ability to enhance transcription of immunoglobulin (Ig) genes in vitro. Paradoxically, OcaB(-/-) mice showed no apparent deficiency in Ig gene transcription, only cellular immune defects including absence of germinal centers (GC) and decreased numbers of immature B cells; the genes targeted by OcaB were not determined. Here we report that OcaB is essential for V(D)J recombination of a subset of IgK genes. We show that OcaB modulates recombination by directly enhancing IgK gene transcription in vivo.

引用

收藏

页码：575 / 585

页数：11

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