Double strand breaks at the HIS2 recombination hot spot in Saccharomyces cerevisiae

Double strand breaks (DSBs) have been found at several meiotic recombination hot spots in Saccharomyces cerevisiae; more global studies have found that they occur at many places along several yeast chromosomes during meiosis, Indeed, the number of breaks found is consistent with the number of recombination events predicted from the genetic map, We have previously demonstrated that the HIS2 gene is a recombination hot spot, exhibiting a high frequency of gene conversion and associated crossing over, This paper shows that DSBs occur in meiosis at a site in the coding region and at a site downstream of the HIS2 gene and that the DSBs are dependent upon genes required for recombination, The frequency of DSBs at HIS2 increases when the gene conversion frequency is increased by alterations in the DNA around HIS2, and vice versa, A deletion that increases both DSBs and conversion ran stimulate both when heterozygous; that is, it is semidominant and acts to stimulate DSBs in trans, These data are consistent with the view that homologous chromosomes associate with each other before the formation of the DSBs.