IL-17 selectively down-regulates TNF-α-induced RANTES gene expression in human colonic subepithelial myofibroblasts

被引：62

作者：

Andoh, A ^{[1
]}

Fujino, S ^{[1
]}

Bamba, S ^{[1
]}

Araki, Y ^{[1
]}

Okuno, T ^{[1
]}

Bamba, T ^{[1
]}

Fujiyama, Y ^{[1
]}

机构：

[1] Shiga Univ Med Sci, Div Gastroenterol, Otsu, Shiga 5202192, Japan

来源：

JOURNAL OF IMMUNOLOGY | 2002年 / 169卷 / 04期

关键词：

D O I：

10.4049/jimmunol.169.4.1683

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IL-17 enhances the TNF-alpha-induced IL-6 and IL-8 secretion in human colonic subepithelial myofibroblasts. In this study, we investigated how IL-17 modulates RANTES secretion in these cells. TNF-alpha potently induced RANTES secretion, but IL-17 dose-dependently inhibited the TNF-alpha-induced RANTES secretion. This was also observed at the mRNA level. Even after pretreatment with TNF-alpha for 12 h, the inhibitory effect of IL-17 was detectable. IL-17 did not affect the TNF-alpha-induced stability of the RANTES gene. IL-17 significantly decreased the TNF-alpha-induced increase in RANTES promoter activity, and IL-17 actually blocked the TNF-alpha-induced RANTES gene transcription. EMSAs demonstrated that IL-17 did not modulate the TNF-alpha-induced NF-kappaB DNA-binding activity, but markedly decreased TNF-alpha-induced IFN regulatory factor-1 (IRF-1) DNA-binding activity. Because cooperation between NF-kappaB and IRF-1 is important in the TNF-alpha-induced RANTES gene expression, the major mechanism mediating the inhibitory effect of IL-17 may be achieved by the inhibition of IRF-1 DNA-binding activity.

引用

页码：1683 / 1687

页数：5

共 24 条

[1] Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-γ- and interleukin-4-induced activation of human keratinocytes [J].

Albanesi, C ;

Scarponi, CS ;

Cavani, A ;

Federici, M ;

Nasorri, F ;

Girolomoni, G .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2000, 115 (01) :81-87

[2] Cytokine regulation of chemokine (IL-8, MCP-1, and RANTES) gene expression in human pancreatic periacinar myofibroblasts [J].

Andoh, A ;

Takaya, H ;

Saotome, T ;

Shimada, I ;

Hata, K ;

Araki, Y ;

Nakamura, F ;

Shintani, Y ;

Fujiyama, Y ;

Bamba, T .

GASTROENTEROLOGY, 2000, 119 (01) :211-219

[3]

Ayad O, 1998, J IMMUNOL, V161, P2594

[4] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[5]

Fossiez F, 1998, Int Rev Immunol, V16, P541, DOI 10.3109/08830189809043008

[6] Chemokine production by the BEAS-2B human bronchial epithelial cells:: Differential regulation of eotaxin, IL-8, and RANTES by TH2-and TH1-derived cytokines [J].

Fujisawa, T ;

Kato, Y ;

Atsuta, J ;

Terada, A ;

Iguchi, K ;

Kamiya, H ;

Yamada, H ;

Nakajima, T ;

Miyamasu, M ;

Hirai, K .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2000, 105 (01) :126-133

[7] Regulation of RANTES chemokine gene expression requires cooperativity between NF-κB and IFN-regulatory factor transcription factors [J].

Génin, P ;

Algarté, M ;

Roof, P ;

Lin, RT ;

Hiscott, J .

JOURNAL OF IMMUNOLOGY, 2000, 164 (10) :5352-5361

[8] IL-17 stimulates inflammatory responses via NF-κB and MAP kinase pathways in human colonic myofibroblasts [J].

Hata, K ;

Andoh, A ;

Shimada, M ;

Fujino, S ;

Bamba, S ;

Araki, Y ;

Okuno, T ;

Fujiyama, Y ;

Bamba, T .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2002, 282 (06) :G1035-G1044

[9]

John M, 1997, J IMMUNOL, V158, P1841

[10] IL-1β induction of RANTES (regulated upon activation, normal T cell expressed and secreted) chemokine gene expression in endometriotic stromal cells depends on a nuclear factor-κB site in the proximal promoter [J].

Lebovic, DI ;

Chao, VA ;

Martini, JF ;

Taylor, RN .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2001, 86 (10) :4759-4764

← 1 2 3 →