Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity

被引：286

作者：

Bredeson, Jessen V. ^{[1
]}

Lyons, Jessica B. ^{[1
]}

Prochnik, Simon E. ^{[2
]}

Wu, G. Albert ^{[2
]}

Ha, Cindy M. ^{[1
,16
]}

Edsinger-Gonzales, Eric ^{[1
,17
]}

Grimwood, Jane ^{[2
,3
]}

Schmutz, Jeremy ^{[2
,3
]}

Rabbi, Ismail Y. ^{[4
]}

Egesi, Chiedozie ^{[5
]}

Nauluvula, Poasa ^{[6
,18
]}

Lebot, Vincent ^{[7
]}

Ndunguru, Joseph ^{[8
]}

Mkamilo, Geoffrey ^{[9
]}

Bart, Rebecca S. ^{[10
]}

Setter, Tim L. ^{[11
]}

Gleadow, Roslyn M. ^{[12
]}

Kulakow, Peter ^{[4
]}

Ferguson, Morag E. ^{[13
]}

Rounsley, Steve ^{[14
]}

Rokhsar, Daniel S. ^{[1
,2
,15
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA

[2] US DOE, Joint Genome Inst, Walnut Creek, CA USA

[3] HudsonAlpha Inst Biotechnol, Huntsville, AL USA

[4] IITA, Ibadan, Nigeria

[5] NRCRI, Umudike, Nigeria

[6] Minist Primary Ind, Koronivia Res Stn, Dept Agr, Suva, Fiji

[7] Ctr Cooperat Int Rech Agron Dev CIRAD, Port Vila, Vanuatu

[8] MARI, Dar Es Salaam, Tanzania

[9] NARI, Mtwara, Tanzania

[10] Donald Danforth Plant Sci Ctr, St Louis, MO USA

[11] Cornell Univ, Sch Integrat Plant Sci, Sect Soil & Crop Sci, Ithaca, NY USA

[12] Monash Univ, Sch Biol Sci, Melbourne, Vic 3004, Australia

[13] IITA, Nairobi, Kenya

[14] Dow AgroSci, Indianapolis, IN USA

[15] Okinawa Inst Sci & Technol Grad Univ, Mol Genet Unit, Onna, Japan

[16] Univ Colorado, Anschutz Med Campus, Denver, CO 80202 USA

[17] Marine Biol Lab, Woods Hole, MA 02543 USA

[18] South Pacific Community, Kolonia, Micronesia

来源：

NATURE BIOTECHNOLOGY | 2016年 / 34卷 / 05期

基金：

美国国家卫生研究院;

关键词：

MANIHOT-ESCULENTA CRANTZ; GENOMIC SELECTION; MANIOC MANIHOT; RESISTANCE; ORIGIN; DOMESTICATION; ACCESSIONS;

D O I：

10.1038/nbt.3535

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cassava (Manihot esculenta) provides calories and nutrition for more than half a billion people. It was domesticated by native Amazonian peoples through cultivation of the wild progenitor M. esculenta ssp. flabellifolia and is now grown in tropical regions worldwide. Here we provide a high-quality genome assembly for cassava with improved contiguity, linkage, and completeness; almost 97% of genes are anchored to chromosomes. We find that paleotetraploidy in cassava is shared with the related rubber tree Hevea, providing a resource for comparative studies. We also sequence a global collection of 58 Manihot accessions, including cultivated and wild cassava accessions and related species such as Ceara or India rubber (M. glaziovii), and genotype 268 African cassava varieties. We find widespread interspecific admixture, and detect the genetic signature of past cassava breeding programs. As a clonally propagated crop, cassava is especially vulnerable to pathogens and abiotic stresses. This genomic resource will inform future genome-enabled breeding efforts to improve this staple crop.

引用

页码：562 / 570

页数：9

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