Inhibition of the binding of SNAP-23 to syntaxin 4 by Munc18c

被引：81

作者：

Araki, S ^{[1
]}

Tamori, Y ^{[1
]}

Kawanishi, M ^{[1
]}

Shinoda, H ^{[1
]}

Masugi, J ^{[1
]}

Mori, H ^{[1
]}

Niki, T ^{[1
]}

Okazawa, H ^{[1
]}

Kubota, T ^{[1
]}

Kasuga, M ^{[1
]}

机构：

[1] KOBE UNIV,SCH MED,DEPT INTERNAL MED 2,CHUO KU,KOBE,HYOGO 650,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 234卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.6560

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SNARE proteins have been implicated in the insulin-induced translocation of vesicles containing the GLUT4 glucose transporter to the plasma membrane of adipocytes. The role of the target SNARE SNAP-25 or its homologs in this process was investigated by screening a mouse adipocyte cDNA library with rat SNAP-25 and human SNAP-23 cDNA probes. Both positive clones isolated encoded a protein with 87% sequence identity to human SNAP-23, and which was therefore designated mouse SNAP-23. Immunoblot and immunofluorescence analyses revealed that SNAP-23 is located predominantly in the plasma membrane of 3T3-L1 adipocytes incubated in the absence or presence of insulin. Of syntaxins 1 to 5, SNAP-23 bound with the highest affinity to syntaxins 1 and 4 in the yeast two-hybrid system. Expression of SNAP-23, syntaxin 4, and the syntaxin-binding protein Munc18c in COS cells revealed that Munc18c reduced the amount of SNAP-23 bound to syntaxin 4 in a concentration-dependent manner. These results suggest that the binding of SNAP-23 to syntaxin 4 is inhibited by Munc18c in adipocytes. (C) 1997 Academic Press.

引用

页码：257 / 262

页数：6

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