Genome sequence of the human malaria parasite Plasmodium falciparum

被引：3374

作者：

Gardner, MJ

Hall, N

Fung, E

White, O

Berriman, M

Hyman, RW

Carlton, JM

Pain, A

Nelson, KE

Bowman, S

Paulsen, IT

James, K

Eisen, JA

Rutherford, K

Salzberg, SL

Craig, A

Kyes, S

Chan, MS

Nene, V

Shallom, SJ

Suh, B

Peterson, J

Angiuoli, S

Pertea, M

Allen, J

Selengut, J

Haft, D

Mather, MW

Vaidya, AB

Martin, DMA

Fairlamb, AH

Fraunholz, MJ

Roos, DS

Ralph, SA

McFadden, GI

Cummings, LM

Subramanian, GM

Mungall, C

Venter, JC

Carucci, DJ

Hoffman, SL

Newbold, C

Davis, RW

Fraser, CM

Barrell, B

机构：

[1] Inst Genom Res, Rockville, MD 20850 USA

[2] Wellcome Trust Genome, Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[3] Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA

[4] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England

[5] Univ Oxford, John Radcliffe Hosp, Weatherall Inst Mol Med, Oxford OX3 9DU, England

[6] Drexel Univ, Coll Med, Dept Microbiol & Immunol, Philadelphia, PA 19129 USA

[7] Univ Dundee, Wellcome Trust Bioctr, Sch Life Sci, Dundee DD1 5EH, Scotland

[8] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

[9] Univ Penn, Genom Inst, Philadelphia, PA 19104 USA

[10] Univ Melbourne, Sch Bot, Plant Res Ctr, Melbourne, Vic 3010, Australia

[11] Celera Genom, Rockville, MD 20850 USA

[12] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley Drosophila Genome Project, Berkeley, CA 94720 USA

[13] Ctr Advancement Genom, Rockville, MD 20850 USA

[14] USN, Med Res Ctr, Malaria Program, Silver Spring, MD 20910 USA

来源：

NATURE | 2002年 / 419卷 / 6906期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/nature01097

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

The parasite Plasmodium falciparum is responsible for hundreds of millions of cases of malaria, and kills more than one million African children annually. Here we report an analysis of the genome sequence of P. falciparum clone 3D7. The 23-megabase nuclear genome consists of 14 chromosomes, encodes about 5,300 genes, and is the most (A + T)-rich genome sequenced to date. Genes involved in antigenic variation are concentrated in the subtelomeric regions of the chromosomes. Compared to the genomes of free-living eukaryotic microbes, the genome of this intracellular parasite encodes fewer enzymes and transporters, but a large proportion of genes are devoted to immune evasion and host-parasite interactions. Many nuclear-encoded proteins are targeted to the apicoplast, an organelle involved in fatty-acid and isoprenoid metabolism. The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria.

引用

页码：498 / 511

页数：14

共 165 条

[1]

Genes for two mitochondrial ribosomal proteins in flowering plants are derived from their chloroplast or cytosolic counterparts [J].

Adams, KL ;

Daley, DO ;

Whelan, J ;

Palmer, JD .

PLANT CELL, 2002, 14 (04) :931-943

[2]

Adisa A, 2001, J CELL SCI, V114, P3377

[3]

A homologue of Sar1p localises to a novel trafficking pathway in malaria-infected erythrocytes [J].

Albano, FR ;

Berman, A ;

La Greca, N ;

Hibbs, AR ;

Wickham, M ;

Foley, M ;

Tilley, L .

EUROPEAN JOURNAL OF CELL BIOLOGY, 1999, 78 (07) :453-462

[4]

The InterPro database, an integrated documentation resource for protein families, domains and functional sites [J].

Apweiler, R ;

Attwood, TK ;

Bairoch, A ;

Bateman, A ;

Birney, E ;

Biswas, M ;

Bucher, P ;

Cerutti, T ;

Corpet, F ;

Croning, MDR ;

Durbin, R ;

Falquet, L ;

Fleischmann, W ;

Gouzy, J ;

Hermjakob, H ;

Hulo, N ;

Jonassen, I ;

Kahn, D ;

Kanapin, A ;

Karavidopoulou, Y ;

Lopez, R ;

Marx, B ;

Mulder, NJ ;

Oinn, TM ;

Pagni, M ;

Servant, F ;

Sigrist, CJA ;

Zdobnov, EM .

NUCLEIC ACIDS RESEARCH, 2001, 29 (01) :37-40

[5]

Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].

Kaul, S ;

Koo, HL ;

Jenkins, J ;

Rizzo, M ;

Rooney, T ;

Tallon, LJ ;

Feldblyum, T ;

Nierman, W ;

Benito, MI ;

Lin, XY ;

Town, CD ;

Venter, JC ;

Fraser, CM ;

Tabata, S ;

Nakamura, Y ;

Kaneko, T ;

Sato, S ;

Asamizu, E ;

Kato, T ;

Kotani, H ;

Sasamoto, S ;

Ecker, JR ;

Theologis, A ;

Federspiel, NA ;

Palm, CJ ;

Osborne, BI ;

Shinn, P ;

Conway, AB ;

Vysotskaia, VS ;

Dewar, K ;

Conn, L ;

Lenz, CA ;

Kim, CJ ;

Hansen, NF ;

Liu, SX ;

Buehler, E ;

Altafi, H ;

Sakano, H ;

Dunn, P ;

Lam, B ;

Pham, PK ;

Chao, Q ;

Nguyen, M ;

Yu, GX ;

Chen, HM ;

Southwick, A ;

Lee, JM ;

Miranda, M ;

Toriumi, MJ ;

Davis, RW .

NATURE, 2000, 408 (6814) :796-815

[6]

Gene Ontology: tool for the unification of biology [J].

Ashburner, M ;

Ball, CA ;

Blake, JA ;

Botstein, D ;

Butler, H ;

Cherry, JM ;

Davis, AP ;

Dolinski, K ;

Dwight, SS ;

Eppig, JT ;

Harris, MA ;

Hill, DP ;

Issel-Tarver, L ;

Kasarskis, A ;

Lewis, S ;

Matese, JC ;

Richardson, JE ;

Ringwald, M ;

Rubin, GM ;

Sherlock, G .

NATURE GENETICS, 2000, 25 (01) :25-29

[7]

GENETIC DIVERSITY OF PLASMODIUM-FALCIPARUM IN A VILLAGE IN EASTERN SUDAN .2. DRUG-RESISTANCE, MOLECULAR KARYOTYPES AND THE MDR1 GENOTYPE OF RECENT ISOLATES [J].

BABIKER, HA ;

CREASEY, AM ;

BAYOUMI, RAL ;

WALLIKER, D ;

ARNOT, DE .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1991, 85 (05) :578-583

[8]

A kingdom-level phylogeny of eukaryotes based on combined protein data [J].

Baldauf, SL ;

Roger, AJ ;

Wenk-Siefert, I ;

Doolittle, WF .

SCIENCE, 2000, 290 (5493) :972-977

[9]

A brief illustrated guide to the ultrastructure of Plasmodium falciparum asexual blood stages [J].

Bannister, LH ;

Hopkins, JM ;

Fowler, RE ;

Krishna, S ;

Mitchell, GH .

PARASITOLOGY TODAY, 2000, 16 (10) :427-433

[10]

Identification of a region of PfEMP1 that mediates adherence of Plasmodium falciparum infected erythrocytes to CD36: Conserved function with variant sequence [J].

Baruch, DI ;

Ma, XC ;

Singh, HB ;

Bi, XH ;

Pasloske, BL ;

Howard, RJ .

BLOOD, 1997, 90 (09) :3766-3775

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