Amoebapore homologs of Caenorhabditis elegans

被引：58

作者：

Bányai, L ^{[1
]}

Patthy, L ^{[1
]}

机构：

[1] Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, H-1518 Budapest, Hungary

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1998年 / 1429卷 / 01期

基金：

匈牙利科学研究基金会;

关键词：

saposin-like domain; amoebapore; NK-lysin; granulysin; (Caenorhabditis elegans);

D O I：

10.1016/S0167-4838(98)00237-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is shown that the Caenorhabditis elegans genome contains several distantly related members of the gene family of saposin-like proteins. The putative products of genes T07C4.4, T08A9.7A, T08A9.7B, T08A9.8, T08A9.9, T08A9.10 are similar to the amoebapores of Entamoeba histolytica, granulysin of cytotoxic T lymphocytes and a putative amoebapore-rerelated protein of the liver fluke Fasciola hepatica inasmuch as they consist of only a single saposin-like domain and a secretory signal peptide. The saposin-like domain of protein T07C4.4, which is most closely related to NK-lysin and granulysin, has been expressed in Escherichia coli and the recombinant protein was shown to have a circular dichroism spectrum consistent with the helix bundle structure characteristic of saposin-like domains. Recombinant T07C4.4 protein was found to have antibacterial activity, suggesting that these amoebapore homologs may pray a role in antibacterial mechanisms of C. elegans. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：259 / 264

页数：6

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